Metabolomics facility

Provided by SYSBIO – Centre of Systems Biology

SYSBIO

METABOLOMICS FACILITY (Free or charged)
With the Metabolomics facility you can explore the metabolic profiles of your samples, identify the most interesting variation between conditions, and see the metabolic flux in action. The Metabolomics Facility includes:
*A complete metabolomics platform (GC-QTOF, LC-QTOF, and specific software)
*Seahorse XF24 Analyzer
*YSI 2950D Biochemistry Analyzer
Each instrument is available for specific and diverse analyses, from deep metabolomics analysis to simpler indication about the most important metabolic species (e.g. lactate, oxygen, glutamine, etc.).

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Fluorescence-Activated Cell Sorting (FACS) Core Unit

Provided by CCRI (Children's Cancer Research Institute)

ZANDR

Fluorescence-Activated Cell Sorting (FACS) Core Unit offers practical and theoretical support for all questions regarding flow cytometry. The laboratory presently has at its disposal three flow cytometers for 15 to 19 parameter cell measurements, one of which is a multi-colour device for cell sorting:
LSR II: 405, 488 & 635 nm lasers, 15 parameters
LSR Fortessa: 405, 488, 561 & 640 nm lasers, 19 parameters
FACSAriaFusion: 405, 488, 561 & 640 nm lasers, 19 parameters
Services also include: Cellular therapeutic agents for clinical routine, i.e.:
Cryopreservation and storage of, and storage logistics for autologous and allogeneic blood products
Thawing for reinfusion
CD3/19 depletion prior to HLA-mismatch stem cell transplantation
Cell manipulations and analyses for DLI
Preparation of MNC from blood for extracorporeal photopheresis (“mini ECP”) for treatment of GvHD

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Sample preparation for structural and molecular biology studie

Provided by INSTRUCT-ERIC

instruct-eric

Services offered by Instruct include
Sample Preparation: Crystallisation, Nanobody Discovery, Protein Production;
Biomolecular Analysis: Imaging, Mass Spectrometry, Molecular Biophysics;
3D Structural Analysis: Electron Microscopy, Magnetic Resonance Techniques, X-ray Techniques;

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Human Phenotype Ontology

Provided by HPO - THE HUMAN PHENOTYPE ONTOLOGY

Human Phenotype Ontology

HPO provides an ontology of medically relevant phenotypes, disease-phenotype annotations, and the algorithms that operate on these to allow large-scale computational analysis of the human phenome. The HPO can be used to support differential diagnostics, translational research, and a number of applications in computational biology by providing the means to compute over the clinical phenotype. The HPO is being used for computational deep phenotyping and precision medicine as well as integration of clinical data into translational research. Although initially focused on rare, mainly Mendelian diseases, the focus of the HPO will be extended to other areas of medicine in coming years.
Tools:
* Exomiser: evaluate variants based on the predicted pathogenicity
*genomiser: analyze genome sequence data for non-coding variants
* phenomizer: rank disease differential diagnosis by clinical features
*profile search: discover diseases with a phenotype profile

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Genomics and Proteomics core facility

Provided by GERMAN CANCER RESEARCH CENTER GENOMICS & PROTEOMICS CORE FACILITY

Genomics and Proteomics Core Facility has integrated Many different services:
The Microarray Unit performs expression profiling of mRNA and miRNA, Genotyping and genome-wide methylation analysis.
High-Throughput sequencing is offered and monoclonal antibodies, stable isogenic recombinant cell lines and a clone and vector repository are available.
Additionally we provide booking of central GPCF equipment like Real Time PCR machines, sample preparation robots or SilverQuant scanning systems or the wetlab wiki, which allows you to share and discuss protocols with your colleagues at DKFZ.

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Sequencing services

Provided by NCCT - NGS COMPETENCE CENTER TÜBINGEN

WGGC

The center offers high-throughput sequencing services on the latest Illumina Next Generation Sequencer NovaSeq6000, Oxford Nanopore Technologies Sequencer PromethION and Pacific Bioscience Sequel.

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Next Generation Sequencing services

Provided by West German Genome Center (WGGC)

WGGC

Centralized Next Generation Sequencing (NGS) services are complemented with existing decentralized NGS and bioinformatics expertise of the WGGC partners. Such expertise covers most aspects of NGS research, encompassing capabilities in human and medical genetics as well as onco and plant genomics.

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High Throughput Screening

Provided by I-STEM

I-STEM

I-Stem has a platform for high-throughput screening of libraries of chemical molecules on embryonic stem cells or iPS with genetic defects.
The laboratory has a high-performance robotic platform with miniaturization and standardization of cellular tests as well as the performance of large-scale screening campaigns (10 000 to 40 000 tests per screening campaign) under sterile and reproducible conditions.

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High Throughput Screening, chemical and target libraries

Provided by PCBIS - PLATFORM OF INTEGRATIVE CHEMICAL BIOLOGY OF STRASBOURG

The Platform of Integrative Chemical Biology of Strasbourg (PCBIS) gives access to High Throughput Screening (HTS), chemical libraries and target libraries to academic and private laboratories. The platform evaluates the ADME properties of active molecules: Ciblothèque

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