High throughput sequencing platforms, consisting of
* Analysis platform (SeqOIA-Gen) for qualification, extraction, preparation and sequencing
* Bioinformatics platform (SeqOIA-IT) for data processing, storage, archiving
* Interpretation platform (SeqOIA-LMG): brings together the expert biologists from the Genomic Medicine Laboratories (LMG) who will validate the reports of the medical analyzes of the SeqOIA Medical Biology laboratory (LBM SeqOIA).
Auragen offers access to very high throughput sequencing to players involved in oncology and in the management of rare diseases. DNA / RNA extraction and sequencing are carried out in the medical biology laboratory located in the premises of the HCL (Hospices Civils de Lyon). The bioinformatics processing of the sequences is carried out on the sites of the CHUGA (CHU Grenoble-Alpes) and the CLB ( Center Léon Bérard ). The data are analyzed and interpreted in the various partner establishments of the GCS by AURAGEN experts and colleagues competent in the patient’s pathology.
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CCG offers various gene expression profiling platforms that may be used for high-throughput production and cross platform validations.
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The CCGA offers a broad range of methods for RNA e.g. RNAseq, Single Cell transcriptomics or isoform sequencing as well as DNA e.g. whole genome sequencing, amplicon sequencing or exome sequencing.
Visit websiteProvided by DRESDEN-CONCEPT GENOME CENTER (DCGC)
Next generation sequencing (NGS) and associated technologies are available to researchers in the area of life sciences on the Dresden Campus of DCGC. It provides strong expertise in the areas of:
(1) Single cell applications;
(2) De novo genome sequencing and assemblies; and
(3) A broad range of short read-based sequencing application.
ESGI provides access to new genomic technologies and analytic tools, including
* DNA analysis
* Long Read analysis
* Transcriptome Analysis
*3D genomics
*single cell analysis
*Epigenomic Analysis
Provided by GERMAN CANCER RESEARCH CENTER GENOMICS & PROTEOMICS CORE FACILITY
Genomics and Proteomics Core Facility has integrated Many different services:
The Microarray Unit performs expression profiling of mRNA and miRNA, Genotyping and genome-wide methylation analysis.
High-Throughput sequencing is offered and monoclonal antibodies, stable isogenic recombinant cell lines and a clone and vector repository are available.
Additionally we provide booking of central GPCF equipment like Real Time PCR machines, sample preparation robots or SilverQuant scanning systems or the wetlab wiki, which allows you to share and discuss protocols with your colleagues at DKFZ.
The center offers high-throughput sequencing services on the latest Illumina Next Generation Sequencer NovaSeq6000, Oxford Nanopore Technologies Sequencer PromethION and Pacific Bioscience Sequel.
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Centralized Next Generation Sequencing (NGS) services are complemented with existing decentralized NGS and bioinformatics expertise of the WGGC partners. Such expertise covers most aspects of NGS research, encompassing capabilities in human and medical genetics as well as onco and plant genomics.
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METABOLOMICS FACILITY (Free or charged)
With the Metabolomics facility you can explore the metabolic profiles of your samples, identify the most interesting variation between conditions, and see the metabolic flux in action. The Metabolomics Facility includes:
*A complete metabolomics platform (GC-QTOF, LC-QTOF, and specific software)
*Seahorse XF24 Analyzer
*YSI 2950D Biochemistry Analyzer
Each instrument is available for specific and diverse analyses, from deep metabolomics analysis to simpler indication about the most important metabolic species (e.g. lactate, oxygen, glutamine, etc.).
